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The use of the fluorescent probe 8‐anilino‐1‐naphthalene sulphonic acid (ANS) as a histochemical stain in plant tissues
Author(s) -
GATES P. J.,
OPARKA K. J.
Publication year - 1982
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11572488
Subject(s) - stain , fluorescence , naphthalene , fluorescence microscope , chemistry , toluidine , staining , microscopy , nuclear chemistry , biology , pathology , organic chemistry , optics , medicine , genetics , physics
. The fluorescent probe 8‐anilino‐l‐naphthalene sulphonic acid (ANS) has been evaluated as a histochemical stain for plant tissues. The wide specificity of the compound for hydrophobic binding sites limits its analytical use, but renders it of considerable value as a general fluorescent stain for use in epi‐illumination fluorescence microscopy. Used in this way it is analogous to the light‐microscope stain toluidine blue. ANS has also been found to be a sensitive vascular tracer.