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Fluorescence microscopy of the endomembrane system of living plant cells
Author(s) -
HARRIS N.,
GATES P.
Publication year - 1984
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11572462
Subject(s) - endomembrane system , endoplasmic reticulum , golgi apparatus , organelle , microbiology and biotechnology , fluorescence recovery after photobleaching , fluorescence microscope , biophysics , live cell imaging , cytoplasm , plant cell , fluorescence , biology , chemistry , cell , biochemistry , membrane , physics , quantum mechanics , gene
The fluorochrome Auramine O has been evaluated as a fluorescent probe for components of the endomembrane system of living plant cells. At 0.001% w/v the compound did not inhibit seedling growth or cytoplasmic streaming but stained the nuclear envelope, endoplasmic reticulum and Golgi apparatus. The three‐dimensional, structural interrelationships of these organelles in living tissues could be resolved after minimal tissue preparation. The method is also a valuable control treatment for use in conjunction with electron microscope fixation procedures. It provides a rapid means of examining dynamic changes in the endomembrane system associated with cell development and differentiation and could have application in monitoring the effects of applied physiological or chemical stress.