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Comparative studies on immunological and molecular properties of phosphoenolpyruvate carboxylase in species of Sedum and Kalanchoë performing crassulacean acid metabolism (CAM)
Author(s) -
MULLER D.,
KLUGE M.,
GROSCHELSTEWART U.
Publication year - 1982
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/1365-3040.ep11572356
Subject(s) - crassulacean acid metabolism , phosphoenolpyruvate carboxylase , crassulaceae , kalanchoe , sedum , context (archaeology) , genus , botany , biochemistry , biology , pyruvate carboxylase , chemistry , enzyme , photosynthesis , paleontology
. PEP‐carboxylase was extracted and partially purified from nine species of the genus Sedum and three species of the genus Kalanchoe, all performing CAM. Immunological and molecular properties of these enzymes were compared. Molecular weight estimation with gradient slab gels showed identical molecular weights of about 232,000 for all PEP‐carboxylases. Ouchlerlony double‐diffusion analysis, immunotitralion and SDS polyacrylamide clectrophoresis indicated the presence of PEP‐c dimers consisting of monomers of MW 105,000 and 115,000. A model of PEP‐c substructure is proposed. The results are discussed in the context of CAM performance in the genus Sedum