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The downside of eDNA as a survey tool in water bodies
Author(s) -
Roussel JeanMarc,
Paillisson JeanMarc,
Tréguier Anne,
Petit Eric
Publication year - 2015
Publication title -
journal of applied ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.503
H-Index - 181
eISSN - 1365-2664
pISSN - 0021-8901
DOI - 10.1111/1365-2664.12428
Subject(s) - environmental dna , sampling (signal processing) , abundance (ecology) , ecology , aquatic ecosystem , invertebrate , biology , sample (material) , aquatic environment , relative species abundance , environmental science , computer science , chemistry , biodiversity , filter (signal processing) , chromatography , computer vision
Summary There is a growing literature on the detection of species‐specific DNA fragments in water to reveal animals' presence in aquatic environments. Analysis of Rees et al . ( Journal of Applied Ecology , 2014, 51, 1450) and additional publications highlight the downside of the method and suggest that some conclusions have to be toned down. Non‐detection of species‐specific DNA fragments in a water sample does not automatically imply the absence of the target species. A positive signal does not necessarily mean the species is present because the environmental DNA (e DNA ) could have been transported or preserved after an animal's death. Detection of aquatic invertebrates is weakly documented. The effect of species abundance on detection efficiency is not always established. Synthesis and applications . Before successfully becoming an efficient tool to assist proper management policies for monitoring of aquatic species, e DNA surveys must implement sampling strategies that allow estimating detection rates and scaling up information from water samples to larger spatial areas. The effects of discharge variations and dendritic organization in running waters require particular consideration.