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cydA , spdC , and mroQ are novel genes involved in the plasma coagulation of Staphylococcus aureus
Author(s) -
Luo Dong,
Wang Wei,
Chen Qiang,
Peng Linfeng,
Hu Xiaomei,
Chen Kaisen
Publication year - 2021
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12922
Subject(s) - biology , complementation , gene , staphylococcus aureus , genetics , microbiology and biotechnology , bacteria , phenotype
Coagulation is a critical pathogenic process in Staphylococcus aureus . Although the agglutination of S. aureus has been studied for a long time, the genes involved in this process are not completely clear. We performed tube agglutination and dynamic turbidimetry tests to identify novel genes involved in reduced plasma coagulation. A total of 15 genes were identified, including coa , clfA , vwbp , saeS , agrA , trpC , spdC , mroQ , cydA , qoxC , sucC , pyrP , menH , threonine aldolase , and truncated transposase for IS1272 . The functions of these genes include bicomponent regulation, membrane transport, energy metabolism and biosynthesis, respectively. cydA , spdC , and mroQ genes were further studied by gene knockout and complementation. Results of gene knockout and complementation and real‐time‐qPCR proved that cydA , spdC , and mroQ genes are necessary for plasma coagulation. Furthermore, the survival ability of 7 day mice decreased significantly when cydA , spdC , and mroQ genes had been knocked out.