Premium
HIV‐1 tracing method of systemic viremia in vivo using an artificially mutated virus pool
Author(s) -
Soper Andrew,
Koyanagi Yoshio,
Sato Kei
Publication year - 2021
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12862
Subject(s) - viremia , biology , virology , spleen , in vivo , virus , lymphatic system , immunology , haematopoiesis , humanized mouse , stem cell , genetics
The appearance of human immunodeficiency virus type 1 (HIV‐1) plasma viremia is associated with progression to symptomatic disease and CD4 + T cell depletion. To locate the source of systemic viremia, this study employed a novel method to trace HIV‐1 infection in vivo. We created JRCSFξ nef , a pool of infectious HIV‐1 (strain JR‐CSF) with highly mutated nef gene regions by random mutagenesis PCR and infected this mutated virus pool into both Jurkat‐CCR5 cells and hematopoietic stem cell‐transplanted humanized mice. Infection resulted in systemic plasma viremia in humanized mice and viral RNA sequencing helped us to identify multiple lymphoid organs such as spleen, lymph nodes, and bone marrow but not peripheral blood cells as the source of systemic viremia. Our data suggest that this method could be useful for the tracing of viral trafficking in vivo.