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Monoclonal antibodies against foot‐and‐mouth disease virus RNA polymerase for detection of virus infection
Author(s) -
Kushwaha Anand,
Behera Subhasmita,
Saminathan Mani,
Sreedharan Krishnan,
Basagoudanavar Suresh,
Manjunatha Reddy G. B.,
Hosamani Madhusudan
Publication year - 2021
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12860
Subject(s) - foot and mouth disease virus , virology , monoclonal antibody , biology , virus , rna , antibody , escherichia coli , aphthovirus , microbiology and biotechnology , immunology , gene , biochemistry
Foot‐and‐mouth disease (FMD) is a major viral disease in farm animals. In the present study, seven monoclonal antibodies (mAbs) were produced against the FMD virus (FMDV)‐encoded RNA‐dependent RNA polymerase (3D protein) and characterized. Screening of mAb reactivity against three overlapping fragments of the 3D protein expressed in Escherichia coli revealed that the binding sites of all the mAbs were confined to the N‐terminal one‐third of the 3D protein. A selected mAb was utilized for detecting FMDV in the infected cell culture and tissues obtained from FMDV‐infected animals.