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Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor
Author(s) -
Onoda Naoki,
Hiramatsu Yukihiro,
Teruya Shihono,
Suzuki Koichiro,
Horiguchi Yasuhiko
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12799
Subject(s) - bordetella pertussis , virulence , biology , bordetella , microbiology and biotechnology , amino acid , complement (music) , regulator , gene , complement system , genetics , bacteria , phenotype , antibody , complementation
An autotransporter of Bordetella pertussis , virulence‐associated gene 8 (Vag8), binds and inactivates the complement regulator, C1 inhibitor (C1‐Inh), and plays a role in evasion of the complement system. However, the molecular interaction between Vag8 and C1‐Inh remains unclear. Here, we localized the minimum region of Vag8 required for interaction with C1‐Inh by examining the differently truncated Vag8 derivatives for the ability to bind and inactivate C1‐Inh. The truncated Vag8 containing amino‐acid residues 102–548, but not 102–479 and 202–648, showed the full activity of intact Vag8, suggesting that the separate 102–202 and 548–648 amino‐acid regions of Vag8 mediate the interaction with C1‐Inh.