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Changes of frequency and expression level of CD161 in CD8 + T cells and natural killer T cells in peripheral blood of patients with systemic lupus erythematosus
Author(s) -
Park Yumi,
Lim Jinsook,
Kim Seon Young,
Kwon Gye Cheol,
Koo Sun Hoe,
Kim Jimyung
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12798
Subject(s) - natural killer t cell , cd8 , biology , immunology , cytotoxic t cell , interleukin 21 , flow cytometry , anti nuclear antibody , t lymphocyte , t cell , zap70 , pathogenesis , antibody , immune system , microbiology and biotechnology , in vitro , biochemistry , autoantibody
Immunologic abnormalities of natural killer (NK) cells and T cells play a role in the pathogenesis of systemic lupus erythematosus (SLE). CD161 is expressed on most of the NK cells and on some T cells. The quantities of CD161‐expressing cells and expression levels of CD161 were analyzed in T cells and NK cells from patients with SLE compared with normal controls. The expression of CD161 on NK cells, NKT cells, CD4 + T cells, and CD8 + T cells in peripheral blood from patients with inactive SLE and active SLE, and from the normal controls group were determined using flow cytometry. The frequency and expression level of CD161 in the lymphocyte subsets and its relationship with the quantity of regulatory T cells, anti‐double stranded DNA antibody, and the titer of antinuclear antibody were evaluated. Both the percentages of the CD161 + subpopulation and the mean fluorescence intensities (MFIs) of CD161 in CD8 + T cells and NKT cells decreased significantly in SLE patients compared with normal controls ( P < .001). The CD161 expression in CD8 + T cells and NKT cells also decreased in the anti‐dsDNA (+) group ( P < .05). The counts of Treg cells were lower in SLE patients and were weakly correlated with the percentage of the CD161 subpopulation ( r = 0.229, P = .016) and the MFIs of CD161 expression in CD8 + T cells ( r = .232, P = .014). The frequencies and levels of CD161 expression on CD8 + T cells and NKT cells were reduced in SLE patients, suggesting that an abnormality of these cells was related to the pathogenesis of SLE.