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Expression of small RNAs of Bordetella pertussis colonizing murine tracheas
Author(s) -
Hiramatsu Yukihiro,
Suzuki Koichiro,
Motooka Daisuke,
Nakamura Shota,
Horiguchi Yasuhiko
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12791
Subject(s) - bordetella pertussis , biology , whooping cough , gene , microbiology and biotechnology , gene expression , bordetella , rna , regulation of gene expression , bacteria , virology , genetics , vaccination
We performed RNA sequencing on Bordetella pertussis , the causative agent of whooping cough, and identified nine novel small RNAs (sRNAs) that were transcribed during the bacterial colonization of murine tracheas. Among them, four sRNAs were more strongly expressed in vivo than in vitro . Moreover, the expression of eight sRNAs was not regulated by the BvgAS two‐component system, which is the master regulator for the expression of genes contributing to the bacterial infection. The present results suggest a BvgAS‐independent gene regulatory system involving the sRNAs that is active during B. pertussis infection.