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An efflux inhibitor of the MacAB pump in Salmonella enterica serovar Typhimurium
Author(s) -
Yamagishi Ami,
Nakano Sohei,
Yamasaki Seiji,
Nishino Kunihiko
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12765
Subject(s) - salmonella enterica , efflux , microbiology and biotechnology , salmonella , biology , multiple drug resistance , serotype , mutant , virulence , antibiotics , bacteria , biochemistry , gene , genetics
Multidrug efflux pumps play an important role in bacterial multidrug resistance by actively excreting antibiotics. The ATP‐binding cassette–type drug efflux pump MacAB was originally reported as a macrolide‐specific pump. MacAB is also known to be required for the virulence of Salmonella enterica serovar Typhimurium following oral infection in mice. Here, we performed a screening of inhibitors of Salmonella MacAB and found a compound that increased the susceptibility of a MacAB‐expressing strain to macrolides. It was previously reported that MacAB is required to resist peroxide‐mediated killing in vitro and that a supernatant of wild‐type Salmonella rescues the growth defect of a macAB mutant in H 2 O 2 . In this study, we also found that the MacAB inhibitor reduced the ability of the supernatant to rescue Salmonella cells in H 2 O 2 . This compound could lead to a better understanding of the function of MacAB.