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Development of a double‐antibody sandwich ELISA for sensitive detection of Yersinia pestis
Author(s) -
Choi SangYoon,
Rhie Gieun,
Jeon Jun Ho
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12751
Subject(s) - yersinia pestis , biotinylation , polyclonal antibodies , streptavidin , biology , monoclonal antibody , microbiology and biotechnology , antibody , biotin , virology , monoclonal , biochemistry , virulence , immunology , gene
We developed a biotin–streptavidin‐based sandwich ELISA for the sensitive and specific detection of Yersinia pestis . In this assay, the F1 capsular protein and Y. pestis were captured by anti‐F1 mouse monoclonal antibody followed by detection with biotinylated‐anti‐F1 rabbit polyclonal antibody and HRP‐conjugated streptavidin. The developed F1 ELISA could detect not only the F1 protein up to 29 and 17 pg/ml but also Y. pestis up to 177.8 and 129.2 CFU/ml in PBS buffer and human serum, respectively. In addition, the F1 ELISA did not show any cross‐reactivity with various proteins and bacterial strains.