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Comparison of Leptospira species isolated from environmental water and soil in Japan
Author(s) -
Masuzawa Toshiyuki,
Uno Rurika,
Matsuhashi Nana,
Yamaguchi Masaya,
Xu Jun,
Nakamura Shuichi
Publication year - 2019
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12741
Subject(s) - biology , leptospira , clade , 16s ribosomal rna , microbiology and biotechnology , ribosomal rna , trimethoprim , antibiotics , bacteria , phylogenetics , gene , genetics , serotype
Leptospira was isolated from environmental water in central Japan using selective medium comprising five antibiotics, namely sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5‐fluorouracil. Of 100 water samples 57 (57%) were culture‐positive and 50 pure cultures were isolated. Of the 50 cultures isolated from water 48 were classified into a saprophytic clade on the basis of 16S ribosomal RNA gene sequences. However, it was previously reported that isolates from soil in Japan belonged to pathogenic, intermediate, and saprophytic clades, the current findings suggest less diversity of Leptospira species in environmental water than that in soil in Japan.