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Lactobacillus plantarum L‐137 upregulates hyaluronic acid production in epidermal cells and fibroblasts in mice
Author(s) -
Nakai Hiroko,
Hirose Yoshitaka,
Murosaki Shinji,
Yoshikai Yasunobu
Publication year - 2019
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12725
Subject(s) - hyaluronic acid , proinflammatory cytokine , tumor necrosis factor alpha , hairless , lactobacillus plantarum , microbiology and biotechnology , downregulation and upregulation , biology , immune system , hyaluronan synthase , chemistry , biochemistry , lactic acid , immunology , inflammation , bacteria , genetics , gene
Heat‐killed Lactobacillus plantarum L‐137 (HK L‐137), an immunobiotic lactic acid bacterium, has been reported to enhance IFN‐γ production through induction of IL‐12. In this study, we investigated the effects of HK L‐137 on skin moisturizing and production of hyaluronic acid (HA), an extracellular matrix associated with the retention of skin moisture. Oral administration of HK L‐137 suppressed the loss of water content in the stratum corneum in hairless mice. Treatment of primary epidermal cells with HK L‐137 increased HA production. Supernatant from immune cells stimulated by HK L‐137, which contained proinflammatory cytokines such as IL‐12, TNF‐α, and IFN‐γ, upregulated HA production and hyaluronan synthase 2 (HAS2) messenger RNA expression by BALB/3T3 fibroblasts via activation of transcription factor nuclear factor κB (NFκB). Although treatment of the supernatant with anti‐TNF‐α antibody (Ab) alone did not inhibit the HA production, combination of anti‐TNF‐α Ab with anti‐IFN‐γ Ab significantly inhibited the HA production. Thus, HK L‐137‐induced IFN‐γ plays a critical role in upregulated HA production in collaboration with TNF‐α. HK L‐137 may be useful for improvement of skin functions such as moisture retention by inducing HA production.