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Structural modification of Escherichia coli lipid A by myristoyltransferase gene from Klebsiella pneumoniae
Author(s) -
Taniguchi Chiho,
Sugawara Takehiro,
Onoue Sakura,
Kawahara Kazuyoshi
Publication year - 2019
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12722
Subject(s) - lipid a , escherichia coli , klebsiella pneumoniae , mutant , biology , myristic acid , biochemistry , microbiology and biotechnology , lauric acid , gene , lipopolysaccharide , lipid metabolism , fatty acid , palmitic acid , endocrinology
Lipid A in lipopolysaccharide (LPS) of Escherichia coli mutant strains was modified by the introduction of myristoyltransferase gene cloned from Klebsiella pneumoniae . When the gene was introduced into the mutant having lipid A containing only 3‐hydroxymyristic acids, it produced lipid A with two additional myristic acids (C 14:0 ). When the same gene was introduced into the mutant with pentaacylated lipid A containing one lauric acid (C 12:0 ), C 12:0 was replaced by C 14:0 . IL‐6‐inducing activity of LPS with modified lipid A structure suggested that C 12:0 in lipid A could be replaced by C 14:0 without changing the immunostimulating activity.