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Issue Information – Cover
Publication year - 2020
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12701
Subject(s) - t7 rna polymerase , biology , plasmid , ribozyme , citation , rna polymerase , cover (algebra) , computational biology , genome , genetics , microbiology and biotechnology , virology , rna , gene , world wide web , computer science , escherichia coli , mechanical engineering , bacteriophage , engineering
Cover photograph : Reverse genetics system for human rotaviruses (HuRVAs). BHK/T7‐9 cells constitutively expressing T7 RNA polymerase are transfected with a set of 11 rescue T7 plasmids encoding a HuRVA genome with a 3‐fold increased quantity ratio of rescue T7 plasmids encoding NSP2 and NSP5 segments. Recombinant HuRVAs can be recovered after propagation using a high trypsin concentration and the roller‐tube culture technique. PT7 and Rib denote the T7 RNA polymerase promoter and hepatitis delta virus ribozyme sequence, respectively. Microbiol Immunol: 64:401–406 . Article link here
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