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Pseudomonas aeruginosa infection stimulates mitogen‐activated protein kinases signaling pathway in human megakaryocytes
Author(s) -
Gopala Krishnan Gopi Krishnan,
Sethumadhavan Aiswarya,
Vellaichamy Pavithra,
Mani Maheswaran
Publication year - 2019
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12685
Subject(s) - biology , signal transduction , kinase , pseudomonas aeruginosa , microbiology and biotechnology , innate immune system , downregulation and upregulation , p38 mitogen activated protein kinases , protein kinase a , mitogen activated protein kinase , immune system , immunology , biochemistry , gene , genetics , bacteria
Pseudomonas aeruginosa is a major cause of nosocomial infections and contributes to higher mortality in hospitalized individuals. Infection by P. aeruginosa triggers host immune response through activation of pathogen recognition receptors, which are present in innate cells. Several studies have reported the mechanism of P. aeruginosa induced innate immunity in multiple cell types. But so far there is no reports on response of megakaryocytes to P. aeruginosa infection. Hence, our aim was to investigate the precise role and signaling mechanism of megakaryocytes during P. aeruginosa infection. In this study, we used Mo7e cells as representatives of human megakaryocyte and found that P. aeruginosa infection induces cytotoxicity in these cells. We further demonstrated that P. aeruginosa infection modulates p38 and extracellular signal regulated kinase pathways in Mo7e cells. Protein expression profiling in P. aeruginosa lipopolysaccharide‐treated Mo7e cells revealed upregulation of importin subunit β and downregulation of metabolic enzymes. Our results suggest that P. aeruginosa infection regulates mitogen‐activated protein kinases signaling pathway and importin in Mo7e cells and that this is a potential mechanism for nuclear translocation of nuclear factor binding near the κ light‐chain gene in B cells and c‐Jun N‐terminal kinases to induce cell cytotoxicity.