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Generation of a purely clonal defective interfering influenza virus
Author(s) -
Yamagata Yutaro,
Muramoto Yukiko,
Miyamoto Sho,
Shindo Keiko,
Nakano Masahiro,
Noda Takeshi
Publication year - 2019
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12681
Subject(s) - biology , virus , virology , helper virus , influenza a virus , viral interference , viral replication , gene , orthomyxoviridae , reverse genetics , h5n1 genetic structure , genetics , genome , infectious disease (medical specialty) , covid-19 , medicine , disease , pathology
Defective interfering (DI) influenza viruses carry a large deletion in a gene segment that interferes with the replication of infectious virus; thus, such viruses have potential for antiviral therapy. However, because DI viruses cannot replicate autonomously without the aid of an infectious helper virus, clonal DI virus stocks that are not contaminated with helper virus have not yet been generated. To overcome this problem, we used reverse genetics to generate a clonal DI virus with a PB2 DI gene, amplified the clonal DI virus using a cell line stably expressing the PB2 protein, and confirmed its ability to interfere with infectious virus replication in vitro. Thus, our approach is suitable for obtaining purely clonal DI viruses, will contribute to the understanding of DI virus interference mechanisms and can be used to develop DI virus‐based antivirals.