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Development of reference material with assigned value for human T‐cell leukemia virus type 1 quantitative PCR in Japan
Author(s) -
Kuramitsu Madoka,
Okuma Kazu,
Nakashima Makoto,
Sato Tomoo,
Sasaki Daisuke,
Hasegawa Hiroo,
Umeki Kazumi,
Kubota Ryuji,
Sasada Keiko,
Sobata Rieko,
Matsumoto Chieko,
Kaneko Noriaki,
Tezuka Kenta,
Matsuoka Sahoko,
Utsunomiya Atae,
Koh KiRyang,
Ogata Masao,
Ishitsuka Kenji,
Taki Mai,
Nosaka Kisato,
Uchimaru Kaoru,
Iwanaga Masako,
Sagara Yasuko,
Yamano Yoshihisa,
Okayama Akihiko,
Miura Kiyonori,
Satake Masahiro,
Saito Shigeru,
Watanabe Toshiki,
Hamaguchi Isao
Publication year - 2018
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12644
Subject(s) - provirus , jurkat cells , virology , biology , leukemia , real time polymerase chain reaction , microbiology and biotechnology , immunology , t cell , genome , gene , genetics , immune system
ABSTRACT Quantitative PCR (qPCR) of human T‐cell leukemia virus type 1 (HTLV‐1) provirus is used for HTLV‐1 testing and for assessment of risk of HTLV‐1‐related diseases. In this study, a reference material was developed for standardizing HTLV‐1 qPCR. Freeze‐dried TL‐Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08–3.49 copies/100 cells. The maximum difference between laboratories was 3.2‐fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.