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Characterization of three strains of Capnocytophaga canis isolated from patients with sepsis
Author(s) -
Suzuki Michio,
Imaoka Koichi,
Haga Yumi,
Mohri Masahiro,
Nogami Ayako,
Shimojima Yoshio,
Irie Yoshimi,
Sugimura Satoru,
Morikawa Shigeru
Publication year - 2018
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12642
Subject(s) - biology , canis , capnocytophaga , microbiology and biotechnology , sepsis , flavobacteriaceae , bacteria , immunology , genetics , ecology , 16s ribosomal rna , bacteroidetes
Capnocytophaga canimorsus and Capnocytophaga cynodegmi , both commensal bacteria in the oral cavities of dogs and cats, are zoonotic pathogens. In particular, C. canimorsus causes sepsis and fatal septic shock. Recently, a novel Capnocytophaga species, C. canis , was isolated from the oral cavities of healthy dogs. It is reportedly oxidase‐negative and therefore considered avirulent in humans. In the present study, three strains of C. canis were isolated from Japanese patients with sepsis. All three strains, HP20001, HP33001 and HP40001, were oxidase‐positive. Nucleotide sequence identities of the 16S rRNA gene of the three strains to the C. canimorsus type strain ATCC35979, C. cynodegmi type strain ATCC49044 and C. canis type strain LMG29146 were 96.9–97.0%, 96.9–97.0% and 99.7–99.8%, respectively. Multi‐locus sequence analysis based on seven house‐keeping genes, dnaJ , fumC, glyA , gyrB , murG , trpB and tuf , revealed that the oxidase‐positive C. canis strains isolated in Japan and oxidase‐negative strains of C. canis from canine oral cavities in Switzerland were clustered in different genetic subgroups. These results indicate that the virulence of C. canis strains in humans is associated with oxidase activity.