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Bifidobacteria alleviate experimentally induced colitis by upregulating indoleamine 2, 3‐dioxygenase expression
Author(s) -
Zhao Li,
Suolang Yangzhen,
Zhou Dandan,
Tang Yu,
Zhang Yan
Publication year - 2018
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12562
Subject(s) - indoleamine 2,3 dioxygenase , cd11c , foxp3 , colitis , western blot , immunology , biology , immunohistochemistry , inflammatory bowel disease , immune system , cancer research , disease , medicine , biochemistry , tryptophan , phenotype , amino acid , gene
ABSTRACT The goal of this study was explore the role of indoleamine 2, 3‐dioxygenase (IDO) in the therapeutic effect of probiotics on inflammatory bowel disease (IBD). Trinitrobenzene sulfonic acid (TNBS) was used to induce colitis in mice and 1‐methyltryptophan (1‐MT) to block expression of IDO. Clinical manifestations and macroscopic and microscopic colonic changes were assessed using a disease activity index (DAI), the Wallace–Keenan, and Curtner scoring systems, respectively. Expression of colonic IDO was detected by western blot. Immunohistochemistry analysis to evaluate numbers of CD11c + cells and expression of IL‐17 and Foxp3 showed that DAI, Wallace–Keenan, and Curtner scores were lower in the Bifidobacteria treatment group than the control group and that the therapeutic effect of Bifidobacteria was blocked by 1‐MT ( P < 0.05). Additionally, Bifidobacteria were found to increase expression of IDO and the numbers of CD11c + cells, CD11c + and IDO double positive cells and Foxp3 + Treg cells, while decreasing the number of IL‐17 + cells ( P < 0.05). The generation of Foxp3 + Treg cells induced by Bifidobacteria was abrogated by 1‐MT ( P < 0.05). These findings study suggest that Bifidobacteria attenuate TNBS‐induced colitis by inducing expression of IDO, which further increases generation of Foxp3 + Treg cells.