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Proteomic profiling of integral membrane proteins associated to pathogenicity in Vibrio parahaemolyticus strains
Author(s) -
PérezAcosta Jesús A.,
MartínezPorchas Marcel,
ElizaldeContreras José M.,
Leyva Juan Manuel,
RuizMay Eliel,
GollasGalván Teresa,
MartínezCórdova Luis R.,
HuertaOcampo José Ángel
Publication year - 2018
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12556
Subject(s) - vibrio parahaemolyticus , biology , virulence , shrimp , microbiology and biotechnology , proteomics , integral membrane protein , membrane protein , bacterial outer membrane , white spot syndrome , computational biology , bacteria , gene , genetics , escherichia coli , membrane , fishery
Vibrio parahaemolyticus has been recognized as the causal agent of early mortality syndrome and is currently considered an emerging shrimp disease causing losses of millions in the aquaculture industry. Integral membrane proteins are widely recognized as pathogenicity factors involved in essential mechanisms for V. parahaemolyticus infection, which makes them attractive as therapeutic targets. However, their physico‐chemical properties and weak expression has resulted in under‐representation of these proteins in conventional bottom‐up proteomics, making integral membrane proteomics a challenging task. Integral membrane proteins from a bacterial strain isolated from the hepatopancreases of white shrimp with early mortality syndrome and identified by 16S rRNA sequencing as V. parahaemolyticus and an ATCC strain that is pathogenic for humans were obtained by a sequential extraction method and subjected to relative quantification and identification by isobaric Tags for Relative and Absolute Quantitation. A homology database search resulted in identification of more than two hundred proteins, 35 of which are recognized as pathogenic factors showed statistically significant differential accumulation between the strains. These proteins are mainly associated with adherence, secretion systems, cell division, transport, lysogenization, movement and virulence. Identification of pathogenicity‐related proteins in V. parahaemolyticus provides valuable information for developing strategies based on molecular mechanisms that inhibit these proteins, which may be useful therapeutic targets for assisting the shrimp and aquaculture industry.