Premium
Molecular cloning, characterization and expression of immunoglobulin D on pathogen challenge and pathogen associated molecular patterns stimulation in freshwater carp, Catla catla
Author(s) -
Banerjee Rajanya,
Patel Bhakti,
Basu Madhubanti,
Lenka Saswati S.,
Paicha Mahismita,
Samanta Mrinal,
Das Surajit
Publication year - 2017
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12534
Subject(s) - biology , immunoglobulin d , catla , microbiology and biotechnology , grass carp , pathogen , virology , antibody , immunology , carp , b cell , fishery , fish <actinopterygii>
The primordial immunoglobulin class, IgD, was the first non‐IgM isotype discovered in teleosts. The crucial roles of IgM and IgZ in imparting systemic and mucosal immunity, respectively, in various fish species have been widely established. However, the putative function of a unique IgD isotype during pathogenic invasions has not been well explored. The present study reports the existence of an IgD ortholog in freshwater carp, Catla catla, and further evaluates its differential expression profile in response to bacterial, parasitic and viral antigenic exposure and pathogen associated molecular patterns (PAMPs) stimulation. The IgD of C. catla ( Cc IgD) cDNA sequence was found to encode 226 amino acids and confirmed homology with heavy chain delta region of Cyprinidae family members. Phylogenetic analysis of Cc IgD exhibited greatest similarity with Ctenopharyngodon idella . qRT‐PCR analysis revealed significant upregulation ( P < 0.001) of IgD gene expression in kidney with respect to other tissues at 24 hr post‐ Aeromonas hydrophila challenge. Cc IgD gene expression in skin was enhanced following Streptococcus uberis infection and in blood following Argulus infection and inactivated rhabdoviral antigen stimulation. Further, the treatment of bacterial and viral products (PAMPs) also triggered significant ( P < 0.05) increases in Cc IgD mRNA expression in kidney. These findings indicate the functional importance of teleost IgD in orchestrating tissue specific neutralization of antigens on stimulation with different pathogens and PAMPs.