Premium
Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect
Author(s) -
Sato Ko,
Watanabe Oshi,
Ohmiya Suguru,
Chiba Fumiko,
Hayashi Masahiro,
Suzuki Tamio,
Kawakami Kazuyoshi,
Nishimura Hidekazu
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12446
Subject(s) - cytopathic effect , human parainfluenza virus , cell culture , isolation (microbiology) , biology , virology , melanoma , virus , oncolytic virus , microbiology and biotechnology , cancer research , genetics
Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens.