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Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts
Author(s) -
Tasaki Takafumi,
Nukuzuma Souichi,
Takegami Tsutomu
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12440
Subject(s) - biology , virology , japanese encephalitis , viral replication , virus , embryonic stem cell , rna , cell culture , autophagy , encephalitis , gene , genetics , apoptosis
ABSTRACT The role of the autophagy adaptor protein p62/SQSTM1 in Japanese encephalitis virus (JEV) replication in mouse embryonic fibroblasts (MEFs) was investigated. Amounts of JEV RNA and E protein were significantly smaller in p62‐deficient cells than wild‐type cells at 24 hr post‐infection (p.i.). JEV RNA quantitation and viral plaque assays showed significant reductions in viral titers in p62‐deficient cell culture fluid. Our results indicate that JEV replication is impaired in p62‐deficient MEFs, suggesting that p62 positively regulates JEV replication in host cells.