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Occurrence and molecular characteristics of antimicrobial resistance of Escherichia coli from broilers, pigs and meat products in Thailand and Cambodia provinces
Author(s) -
Trongjit Suthathip,
Angkittitrakul Sunpetch,
Chuanchuen Rungtip
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12407
Subject(s) - biology , gene cassette , escherichia coli , broiler , plasmid , antibiotic resistance , microbiology and biotechnology , multiple drug resistance , antimicrobial , gene , veterinary medicine , antibiotics , genetics , integron , zoology , medicine
Nine hundred and forty‐one samples were collected in Sa Keao, Thailand ( n = 554) and Banteay Meanchey, Cambodia ( n = 387) from July 2014 to January 2015. A total of 667 Escherichia coli isolates (381 isolates from Sa Keao and 286 isolates from Banteay Meanchey) were obtained and examined for antimicrobial susceptibility, class 1 integrons, ESBL genes and horizontal transfer of resistance determinants. Prevalence of E. coli in pig and broiler carcass samples from slaughterhouses and fresh markets was 36–85% in Sa Keao and 11–69% in Banteay Meanchey. The majority of these isolates were multidrug resistant (75.3%). Class 1 integrons were common in both Thai (47%) and Cambodian (62%) isolates, of which four resistance gene cassette arrays including aadA1 , dfrA1‐aadA1 , dfrA12‐aadA2 and aadA2‐linF were identified. Class 1 integrons in two broiler isolates from Sa Keao ( dfrA12‐aadA2 ) and one broiler isolate from Banteay Meanchey ( dfrA1‐aadA1 ) were horizontally transferable. Sixteen isolates were confirmed to be ESBL‐producing strains with ESBL gene bla CTX‐M‐15 , broad spectrum β‐lactamase gene bla TEM‐1 and the AmpC gene bla CMY‐2 being detected. The bla TEM‐1 gene was most prevalent and located on a conjugative plasmid.