Premium
Intranasal immunization with a peptide conjugated to Salmonella flagellin induces both systemic and mucosal peptide‐specific antibody responses in mice
Author(s) -
Qian Feng,
Yin Jian,
Li Mengmeng,
Guo Aihua,
Li Ting,
Zhou Ling,
Wu Xin,
Xu Huji
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12396
Subject(s) - flagellin , adjuvant , salmonella enterica , epitope , microbiology and biotechnology , biology , antibody , salmonella , nasal administration , peptide , antigen , virology , immunology , bacteria , receptor , biochemistry , genetics
In this study, the mucosal adjuvant activity of Salmonella flagellin as a carrier in a conjugate of EXP153–rFliC was investigated. EXP153–rFliC was made by conjugation of a synthetic B‐cell epitope peptide derived from Plasmodium falciparum exported protein‐1(EXP153) to recombinant phase 1 flagellin of Salmonella enterica serovar Typhimurium expressed in Escherichia coli (rFliC), and used to immunize BALB/c mice via intranasal instillation. It was found that robust EXP153‐specific serum IgG antibodies were induced without additional adjuvant. EXP153‐specific sIgA antibodies were also induced, these being detected in bronchoalveolar, nasal, vaginal and intestinal washes. These observations demonstrate that Salmonella flagellin as a carrier is an effective mucosal adjuvant in that its conjugated peptide induces antibody responses.