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Proliferation of functional human natural killer cells with anti‐HIV‐1 activity in NOD/SCID/Jak3 null mice
Author(s) -
Hattori Shinichiro,
Matsuda Kouki,
Kariya Ryusho,
Harada Hideki,
Okada Seiji
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12355
Subject(s) - biology , k562 cells , peripheral blood mononuclear cell , interleukin 12 , immunology , cytotoxicity , nod , interleukin 21 , janus kinase 3 , immune system , innate immune system , natural killer cell , lymphokine activated killer cell , cytotoxic t cell , in vitro , virology , t cell , in vivo , leukemia , biochemistry , microbiology and biotechnology
Natural killer cells, a critical component of the innate immune system, eradicate both virus‐infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective proliferation model of functional human NK cells was established in NOD/SCID/Jak3 null (NOJ) mice transplanted with peripheral blood mononuclear cells (PBMC) and K562 cells. The antiviral effects of NK cells were evaluated by challenging this mouse model with HIV‐1. The percentage of intracellular p24 + T cells and the amount of plasma p24 was decreased compared with NOJ mice transplanted with PBMC. Our findings indicate that NK cells have an anti‐HIV‐1 effect through direct cytotoxicity against HIV‐1‐infected cells. These mice provide an important model for evaluating human NK function against human infectious diseases such as HIV‐1 and malignancies.