Premium
The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8 + T‐cell epitopes for active pulmonary tuberculosis
Author(s) -
Duan Zhiliang,
Li Dezhou,
Jia Qingjun,
Xu Juanjuan,
Chen Xinyu,
Xu Zhigang,
Liu Huifang,
Chen Bokun,
Wen Jinsheng
Publication year - 2015
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12339
Subject(s) - epitope , biology , cytotoxic t cell , human leukocyte antigen , cd8 , tuberculosis , t cell , virology , mycobacterium tuberculosis , immunology , antigen , immune system , microbiology and biotechnology , medicine , in vitro , biochemistry , pathology
ABSTRACT MPT63 protein is found only in Mycobacterium tuberculosis complex, including M. tuberculosis and M. bovis . Detection of MPT63‐specific IFN‐γ‐secreting T cells could be useful for the diagnosis of tuberculosis (TB) diseases. In the present study, the HLA‐A*0201 restriction of ten predicted MPT63‐derived CD8 + T‐cell epitopes was assessed on the basis of T2 cell line and HLA‐A*0201 transgenic mice. The diagnostic potential of immunogenic peptides in active pulmonary TB patients was evaluated using an IFN‐γ enzyme‐linked immunospot assay. It was found that five peptides bound to HLA‐A*0201 with high affinity, whereas the remaining peptides exhibited low affinity for HLA‐A*0201. Five immunogenic peptides (MPT63 18–26 , MPT63 29–37 , MPT63 20–28 , MPT63 5–14 and MPT63 10–19 ) elicited large numbers of cytotoxic IFN‐γ‐secreting T cells in HLA‐A*0201 transgenic mice. Each of the five immunogenic peptides was recognized by peripheral blood mononuclear cells from 45% to 73% of 40 HLA‐A*0201 positive TB patients. The total diagnostic sensitivity of the five immunogenic peptides was higher than that of a T‐SPOT. TB assay (based on ESAT‐6 and CFP‐10) (93% versus 90%). It is noticeable that the diagnostic sensitivity of the combination of five immunogenic peptides and T‐SPOT. TB assay reached 100%. These MPT63‐derived HLA‐A*0201‐restricted CD8 + T‐cell epitopes would likely contribute to the immunological diagnosis of M. tuberculosis infection and may provide the components for designing an effective TB vaccine.