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Mannan‐binding lectin at supraphysiological concentrations inhibits differentiation of dendritic cells from human CD14 + monocytes
Author(s) -
Xu XiaoYing,
Li HuiJie,
Zhang LiYun,
Lu Xiao,
Zuo DaMing,
Shan GuiQiu,
Xu TianYu,
Chen ZhengLiang
Publication year - 2015
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12337
Subject(s) - biology , cd14 , mannan binding lectin , monocyte , immune system , major histocompatibility complex , microbiology and biotechnology , immunology , cd80 , lectin , cd40 , biochemistry , cytotoxic t cell , in vitro
ABSTRACT Mannan‐binding lectin (MBL), a circulating C‐type lectin, is an important member of the defense collagen family. It exhibits a high potential for recognizing broad categories of pathogen‐associated molecular patterns and initiating complement cascade responses. DCs are well‐known specialist antigen‐presenting cells that significantly trigger specific T cell‐mediated immune responses. In our previous study, it was observed that high concentrations of MBL significantly attenuate LPS‐induced maturation of monocyte‐derived DCs (MoDCs). In the current study, it was postulated that MBL at similar supraphysiological concentrations would affect early differentiation of MoDCs in some way. CD14 + monocytes from human peripheral blood mononuclear cells were cultured with granulocyte‐macrophage colony‐stimulating factor and IL‐4 in the presence or absence of physiological (1 µg/mL) and supraphysiological concentrations (20 µg/mL) of MBL protein, respectively. Phenotypic analysis indicated that the differentiated DCs incubated with high concentrations of MBL expressed MHC class II and costimulatory molecules (e.g., CD80 and CD40) more weakly than did control groups. The secretion of IL‐10 and IL‐6 increased markedly, whereas their mixed lymphocyte reaction‐stimulating capacity decreased. Members of the signal transducer and activator of transcription family were also found to be differentially regulated. Thus, beyond the role of MBL as an opsonin, our data reveal a possible inhibitory effect of MBL at high concentrations in monocyte‐DC transition, which probably provides one way of regulating adaptive immune responses by strict regulation of DCs, making MBL a better prospect for controlling relevant pathological events such as autoimmune diseases.