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Issue Information – Cover
Publication year - 2016
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12302
Subject(s) - dapi , cover (algebra) , confocal , biology , confocal microscopy , citation , computer science , computational biology , physics , library science , microbiology and biotechnology , genetics , staining , optics , mechanical engineering , engineering
Confocal microscopy of the interaction of human monocyte derived dendritic cells (moDC) with Leishmania mexicana promastigotes. moDC were adhered to poly‐L‐Lysine coverslips and infected with CFDA‐stained promastigotesfor 3 h. Infected‐moDC were stained with Dil to label cell membranes, counterstained with DAPI and analyzed by confocal microscopy. Fluorescence‐positive cells were counted at three random fields (about 100 cells in total) with Huygens Professional software, version 4.2 (Scientific Volume Imaging). moDC are stained in red, nuclei in blue, green parasites were considered inside moDC and green‐red (yellow) parasites were considered outside the cells. Microbiol Immunol : 60:369–381. Article link here .