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Characterization of IS 26 ‐composite transposons and multidrug resistance in conjugative plasmids from Enterobacter cloacae
Author(s) -
Chen ChihMing,
Yu WenLiang,
Huang Mei,
Liu JauJin,
Chen IChien,
Chen HueiFen,
Wu LiiTzu
Publication year - 2015
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12289
Subject(s) - biology , enterobacter cloacae , plasmid , integron , mobile genetic elements , genetics , multiple drug resistance , transposable element , microbiology and biotechnology , gene cassette , locus (genetics) , gene , escherichia coli , drug resistance , enterobacteriaceae , genome
SHV‐12 is the most widespread resistance determinant of Enterobacter cloacae in Taiwan; however, bla SHV‐12 has rarely been mobilized. Six multidrug‐resistant E. cloacae isolates were collected. After conjugal transfer, plasmid profiling and analysis of incompatibility groups was performed to characterize the genetic context of bla SHV‐12 ‐containing fragments. The presence of mobile genetic elements was demonstrated by PCR, cloning, sequencing and bioinformatics analyses. Four different β‐lactamase genes ( bla TEM‐1 , bla SHV‐12 , bla CTX‐M‐3 and/or bla CTX‐M‐14 ) were observed in the conjugative plasmids belonging to the IncHI2 ( n = 4), IncI1 or IncP incompatibility groups. The IS 26‐bla SHV‐12 ‐IS 26 locus was located in five different genetic environments. A novel structural organization of a class 1 integron with the aac(6')‐IIc cassette truncated by IS 26 was identified in one isolate. Thus, bla SHV‐12 was obtained from different plasmids through IS 26 ‐mediated homologous recombination. IS 26 plays a vital role in the distribution of mobile resistance elements between different plasmids found in multidrug‐resistant E. cloacae isolates.