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Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTX Vv as a potential vaccine for Vibrio vulnificus
Author(s) -
Lee Tae Hee,
Chung Kyung Min
Publication year - 2015
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12284
Subject(s) - vibrio vulnificus , biology , virulence , vibrio infections , cysteine protease , microbiology and biotechnology , protease , immunization , virology , vibrionaceae , bacteria , immune system , enzyme , genetics , biochemistry , gene
Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus . However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPD Vv (C3725S), of the well‐conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats‐in‐toxin (MARTX Vv /RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPD Vv (C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.