Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTX Vv  as a potential vaccine for  Vibrio vulnificus | Zendy

Lee Tae Hee | Zendy; Chung Kyung Min | Zendy

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Development and characterization of a catalytically inactive cysteine protease domain of RtxA1/MARTX Vv as a potential vaccine for Vibrio vulnificus

Author(s) -

Lee Tae Hee,

Chung Kyung Min

Publication year - 2015

Publication title -

microbiology and immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.664

H-Index - 70

eISSN - 1348-0421

pISSN - 0385-5600

DOI - 10.1111/1348-0421.12284

Subject(s) - vibrio vulnificus , biology , virulence , vibrio infections , cysteine protease , microbiology and biotechnology , protease , immunization , virology , vibrionaceae , bacteria , immune system , enzyme , genetics , biochemistry , gene

Recent studies have defined several virulence factors as vaccine candidates against Vibrio vulnificus . However, most of these factors have the potential to cause pathogenic effects in the vaccinees or induce incomplete protection. To overcome these drawbacks, a catalytically inactive form, CPD Vv (C3725S), of the well‐conserved cysteine protease domain (CPD) of V. vulnificus multifunctional autoprocessing repeats‐in‐toxin (MARTX Vv /RtxA1) was recombinantly generated and characterized. Notably, active and passive immunization with CPD Vv (C3725S) conferred protective immunity against V. vulnificus strains. These results may provide a novel framework for developing safe and efficient subunit vaccines and/or therapeutics against V. vulnificus that target the CPD of MARTX toxins.

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