MDCK cells were infected with influenza A virus and were fixed at 7 h postinfection. The cells were subjected to indirect immunostaining with anti‐alpha‐tubulin monoclonal antibody (clone DM1A, Sigma‐Aldrich) and Alexa Fluor 488‐conjugated anti‐mouse Ig. Following post‐fixation, the cells were directly immunostained with Alexa Fluor 568‐conjugated anti‐NP monoclonal antibody (mAb61A5) which preferentially recognized viral RNP complexes. Confocal microscopy revealed that the viral RNPs, most likely on recycling endosomes (red), were localized along the microtubules (green). Nuclei were stained with TO‐PRO‐3 (blue)