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Induction of potential protective immunity against enterotoxemia in calves by single or multiple recombinant Clostridium perfringens toxoids
Author(s) -
Jiang Zhigang,
De Yanyan,
Chang Jitao,
Wang Fang,
Yu Li
Publication year - 2014
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12198
Subject(s) - toxoid , clostridium perfringens , recombinant dna , microbiology and biotechnology , biology , virology , vaccination , antibody , immunology , tetanus , bacteria , biochemistry , genetics , gene
Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridial vaccines is dangerous, expensive, and time‐consuming, the prospect of recombinant toxoid vaccines against diseases caused by C. perfringens toxins is promising. In this study, nontoxic recombinant toxoids derived from α‐, β‐ and ε‐toxins of C. perfringens , namely, rCPA 247–370 , rCPB and rEtxHP, respectively, were expressed in Escherichia coli . High levels of specific IgG antibodies and neutralizing antibodies against the toxins were detected in sera from calves vaccinated with either a single recombinant toxoid or a mixed cocktail of all three recombinant toxoids, indicating the potential of these recombinant toxoids to provide calves with protective immunity against enterotoxemia caused by C. perfringens .