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Protective immunity by oral immunization with heat‐killed Shigella strains in a guinea pig colitis model
Author(s) -
Barman Soumik,
Koley Hemanta,
Ramamurthy Thandavarayan,
Chakrabarti Manoj Kumar,
Shinoda Sumio,
Nair Gopinath Balakrish,
Takeda Yoshifumi
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12095
Subject(s) - shigella , shigella flexneri , shigella boydii , microbiology and biotechnology , shigella sonnei , shigella dysenteriae , shigellosis , serotype , biology , virology , salmonella , bacteria , escherichia coli , biochemistry , genetics , gene
The protective efficacy of and immune response to heat‐killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains ( Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei ) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1 × 10 7 of each serogroup/serotype of heat‐killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1 × 10 9 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat‐killed cells of the six Shigella serogroups/serotypes studied would be a possible broad‐spectrum candidate vaccine against shigellosis.