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Kinetic studies of the effect of a 17‐nucleotide difference in the 0.3‐kb region containing the R‐ U 5‐5′ leader sequence of Friend murine leukemia virus on viral gene expression
Author(s) -
Choo Yeng Cheng,
Seki Yohei,
TakaseYoden Sayaka
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12072
Subject(s) - biology , clone (java method) , murine leukemia virus , recombinant dna , microbiology and biotechnology , virology , messenger rna , nucleic acid sequence , gene , virus , nucleotide , genetics
In addition to the env gene, a 0.3‐kb fragment containing the R‐U5‐5′ leader sequence is essential for the induction of spongiform neurodegeneration by Friend murine leukemia virus (Fr‐MLV) clone A8 and it also influences expression of the Env protein. Kinetic studies were carried out using two recombinant viruses, R7f, carrying the A8 0.3‐kb fragment, and Rec5, carrying the 0.3‐kb fragment of the non‐neuropathogenic Fr‐MLV clone 57. These analyses suggested that the 0.3‐kb fragment influenced the expression level of the Env protein by regulating the amount of spliced env ‐mRNA rather than the amount of total viral mRNA or viral production.