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Identification of cellular factors required for the budding of koala retrovirus
Author(s) -
Shimode Sayumi,
Nakaoka Rie,
Hoshino Shigeki,
Abe Masumi,
Shogen Hiroko,
Yasuda Jiro,
Miyazawa Takayuki
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12066
Subject(s) - biology , retrovirus , gammaretrovirus , tsg101 , escrt , budding , endosome , ubiquitin , microbiology and biotechnology , virology , genetics , gene , virus , microrna , microvesicles , intracellular
Koala retrovirus (KoRV) is a unique gammaretrovirus that is currently endogenizing into its host and considered to be associated with leukemia, lymphoma and immunosuppression in koalas ( Phascolactos cinereus ). In this study, it was demonstrated that WWP2 or WWP2‐like E3 ubiquitin ligases possessing the WW domain closely related to WWP2 and Vps4A/B are involved in KoRV budding. These data suggest that KoRV Gag recruits the cellular endosomal sorting complex required for transport machinery through interaction of the PPPY L ‐domain with the WW domain(s) of WWP2 and that progeny virions are released from cells by utilizing the multivesicular body sorting pathway.