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Expression and immunological characterization of cardamom mosaic virus coat protein displaying HIV gp41 epitopes
Author(s) -
Damodharan Subha,
Gujar Ravindra,
Pattabiraman Sathyamurthy,
Nesakumar Manohar,
Hanna Luke Elizabeth,
Vadakkuppattu Ramanathan D,
Usha Ramakrishnan
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12045
Subject(s) - gp41 , epitope , biology , virology , virus , coat protein , fusion protein , tobacco mosaic virus , antigen , peripheral blood mononuclear cell , escherichia coli , microbiology and biotechnology , immunology , biochemistry , rna , gene , in vitro , recombinant dna
The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus , assembles into virus‐like particles when expressed in an Escherichia coli expression system. The N and C‐termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric proteins reacted with sera from HIV positive persons and also stimulated secretion of cytokines by peripheral blood mononuclear cells from these persons. Thus, a system based on the coat protein of CdMV can be used to display HIV‐1 antigens.