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Rapid detection of human cytomegalovirus UL 97 and UL 54 mutations for antiviral resistance in clinical specimens
Author(s) -
Daikoku Tohru,
Saito Kazuhide,
Aihara Takamitsu,
Ikeda Masahiro,
Takahashi Yoshiyuki,
Hosoi Hiroki,
Nishida Tetsuya,
Takemoto Masaya,
Shiraki Kimiyasu
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12043
Subject(s) - biology , human cytomegalovirus , virology , cytomegalovirus , genotype , drug resistance , gene , mutation , virus , polymerase chain reaction , antiviral drug , microbiology and biotechnology , herpesviridae , viral disease , genetics
Drug‐resistant cytomegalovirus appears during prolonged anti‐cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.