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Sequence and phylogenetic analyses of the water‐soluble glucan synthesizing‐glucosyltransferase genes of Streptococcus dentirousetti
Author(s) -
ShinozakiKuwahara Noriko,
Saito Masanori,
Hirasawa Masatomo,
Takada Kazuko
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12040
Subject(s) - glucosyltransferase , streptococcus sobrinus , biology , open reading frame , amino acid , gene , peptide sequence , phylogenetic tree , glucan , sequence analysis , biochemistry , genetics , microbiology and biotechnology , bacteria , streptococcus mutans
Two tandemly aligned glucosyltransferase (GTF) genes whose gene products are responsible for water‐soluble glucan synthesis were isolated from Streptococcus dentirousetti NUM1303 and sequenced. One of the GTF genes of S. dentirousetti consisted of a 4110 bp open reading frame (ORF) that encoded for a 1369 amino acid protein and was revealed to be a S. sobrinus gtfS homolog. The percent similarity of amino acid sequences of the GTF‐S from S. dentirousetti compared to those from S. sobrinus was 99%. In addition, a putative gtfT was found in tandem in the downstream region of the S. dentirousetti gtfS . The gtfT of S. dentirousetti consisted of a 4527 bp ORF encoding for 1508 amino acids. The similarity of amino acid sequences of the GTF‐T from S. dentirousetti and S. sobrinus was 94%. Phylogenetic analysis based on amino acid sequences from other related streptococcal GTFs suggested that both GTF‐S and GTF‐T of S. dentirousetti are closely related to S. sobrinus .