Premium
Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae
Author(s) -
Morita Masatomo,
Yamamoto Shouji,
Hiyoshi Hirotaka,
Kodama Toshio,
Okura Masatoshi,
Arakawa Eiji,
Alam Munirul,
Ohnishi Makoto,
Izumiya Hidemasa,
Watanabe Haruo
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12039
Subject(s) - vibrio cholerae , biology , horizontal gene transfer , gene , genetics , genome , pathogenicity island , cholera , pulsed field gel electrophoresis , gene cluster , vibrionaceae , microbiology and biotechnology , bacteria , genotype
Twelve Vibrio cholerae isolates with genes for a type III secretion system (T3SS) were detected among 110 environmental and 14 clinical isolates. T3SS‐related genes were distributed among the various serogroups and pulsed‐field gel electrophoresis of Not I‐digested genomes showed genetic diversity in these strains. However, the restriction fragment length polymorphism profiles of the T3SS‐related genes had similar patterns. Additionally, naturally competent T3SS‐negative V. cholerae incorporated the ca. 47 kb gene cluster of T3SS, which had been integrated into a site on the chromosome by recombination. Therefore, it is suggested that horizontal gene transfer of T3SS‐related genes occurs among V. cholerae in natural ecosystems.