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Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection
Author(s) -
Waters Stephanie,
Luther Sanjana,
Joerger Torsten,
Richards Gary P.,
Boyd E. Fidelma,
Parent Michelle A.
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12034
Subject(s) - vibrio parahaemolyticus , biology , microbiology and biotechnology , immune system , cytokine , cd86 , immunology , pathogen , tlr2 , immunity , macrophage , innate immune system , t cell , bacteria , biochemistry , genetics , in vitro
Vibrio parahaemolyticus is the most common cause of bacterial, seafood‐related illness in the USA. Currently, there is a dearth of published reports regarding immunity to infection with this pathogen. Here, production of both pro‐ and anti‐inflammatory cytokines by V. parahaemolyticus‐ infected RAW 264.7 murine macrophages was studied. It was determined that this infection results in increased concentrations of IL‐1α, IL‐6, TNF‐α and IL‐10. Additionally, decreases in cell surface TLR2 and TLR4 and increases in T‐cell co‐stimulatory molecules CD40 and CD86 were discovered. The data presented here begin to identify the immune variables required to eliminate V. parahaemolyticus from infected host tissues.