Premium
Simple method for differentiating measles vaccine from wild‐type strains using loop‐mediated isothermal amplification
Author(s) -
Nakayama Tetsuo,
Sawada Akihito,
Kubo Hideyuki,
Kaida Atsushi,
Tanaka Toshimitsu,
Shigemoto Naoki,
Komase Katsuhiro,
Takeda Makoto
Publication year - 2013
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/1348-0421.12029
Subject(s) - loop mediated isothermal amplification , virology , biology , measles virus , primer (cosmetics) , measles vaccine , measles , wild type , microbiology and biotechnology , vaccination , gene , genetics , chemistry , dna , mutant , organic chemistry
Because of increasing measles vaccine coverage, the proportion of patients with modified measles has been increasing. Such patients have low‐grade fever with very mild eruptions similar to vaccine‐related adverse events. Differentiation between these two pathogenic conditions is required to improve the quality of laboratory‐based measles surveillance. In this study, vaccine‐specific and wild‐type specific primer sets were designed for loop‐mediated isothermal amplification in the N gene, and vaccine strains, C1, D3, D4, D5, D8, D9, G3 and H1 wild strains were examined. Three vaccine strains were efficiently amplified using a vaccine‐specific primer set with an approximately 10‐times higher sensitivity than wild‐type primer. Modified measles was differentiated from vaccine‐associated cases by this system, but limitations were encountered with the other genotypes.