Premium
The expression of cell adhesion molecule 1 and its splicing variants in Sézary cells and cell lines from cutaneous T‐cell lymphoma
Author(s) -
Yamaguchi Mari,
Morizane Shin,
Hamada Toshihisa,
Miyake Tomoko,
Sugaya Makoto,
Iwata Hiroaki,
Fujii Kazuyasu,
HaramotoShiratsuki Rie,
Nakagawa Yuki,
Miura Mayumi,
Ohshima Koichi,
Morishita Kazuhiro,
Takahashi Takahide,
Imada Masahide,
Okada Ken,
Uehara Jiro,
SowaOsako Junko,
Iwatsuki Keiji
Publication year - 2019
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.15078
Subject(s) - mycosis fungoides , lymphoma , anaplastic large cell lymphoma , microbiology and biotechnology , cell culture , cutaneous t cell lymphoma , cell adhesion molecule , cancer research , biology , cell , immunology , biochemistry , genetics
Cell adhesion molecule 1 ( CADM 1) is aberrantly expressed by T‐cell neoplasms such as adult T‐cell leukemia/lymphoma ( ATLL ) and mycosis fungoides ( MF ). We studied the expression of CADM 1 and its splicing variants in Sézary syndrome ( SS ), MF , other cutaneous T‐cell lymphoma ( CTCL ), and cell lines derived from T‐ and B‐cell lymphomas. Soluble CADM 1 was measured in the patients’ sera. CADM 1+ cells in the blood and skin lesions were examined by flow cytometry and immunostaining, respectively. Soluble CADM 1 was measured by ELISA , and the splicing variants of CADM 1 transcripts were determined by reverse transcriptase‐polymerase chain reaction, followed by sequencing. As a result, circulating CADM 1+ cells were significantly increased in seven out of 10 patients with SS , ranging from 7.9% to 74.5% of the CD 3+ CD 4+ fractions (median 33.7%; cut‐off value 6.5%). The percentages of CADM 1+ cells were usually less than those of circulating Sézary cells. CADM 1 was expressed, to various degrees, in six of nine T‐cell lines derived from SS , MF , ATLL , and anaplastic large cell lymphoma ( ALCL ), but negative in B‐cell lymphoma‐derived cell lines. CADM 1+ cells were present in the skin infiltrates of MF , SS , ATLL and ALCL . Serum levels of soluble CADM 1 were not significantly elevated in SS / MF . Three major splicing variants of CADM 1 expressed by neoplastic T‐cells contained different combinations of the exons 7, 8, 9 and 11, including a putative oncogenic variant composed of exons 7‐8‐9‐11. In conclusion, CADM 1 is frequently expressed in Sézary cells and cell lines from CTCL .