Premium
Claudin reduction may relate to an impaired skin barrier in rosacea
Author(s) -
Deng Zhili,
Chen Mengting,
Xie Hongfu,
Jian Dan,
Xu San,
Peng Qinqin,
Sha Ke,
Liu Yingzi,
Zhang Yiya,
Shi Wei,
Li Ji
Publication year - 2019
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.14792
Subject(s) - skin barrier , rosacea , claudin , reduction (mathematics) , chemistry , dermatology , medicine , biology , tight junction , microbiology and biotechnology , mathematics , acne , geometry
Rosacea is a chronic inflammatory skin disorder whose pathophysiological mechanism remains largely unknown. Although recent studies have revealed the hypersensitivity of the skin towards chemical, thermal and biological stimuli, there is no direct molecular evidence suggesting the skin barrier is impaired in rosacea. In this study, we demonstrated that the mRNA levels of most claudins ( CLDN ), the main components of tight junctions determining the major barrier of the paracellular pathway between epithelial cells, were lowered in lesional skin of rosacea patients, especially with erythematotelangiectatic ( ETR ) and papulopustular ( PPR ) subtypes. Immunohistochemical analysis showed a significant decrease in the expression of CLDN 1, CLDN 3, CLDN 4 and CLDN 5 in the epidermis of ETR and PPR patients. However, the expression of other skin barrier genes, such as filaggrin, loricrin and keratin 10, was not altered. In vitro , various rosacea trigger factors reduced the protein levels of CLDN 1, CLDN 3 and CLDN 5 in keratinocytes. Taken together, our results demonstrate a significant decrease in the expression of CLDN rather than other skin barrier genes, which may be associated with an impaired skin barrier responsible for the development of rosacea.