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Pilot study on the correlation between dermoscopic patterns and fluorescence in situ hybridization findings using whole‐slide digital imaging for acral volar melanocytic lesions
Author(s) -
Ogata Dai,
Arai Eiichi,
Goto Yoshiya,
Tsuchida Tetsuya
Publication year - 2018
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.14324
Subject(s) - h&e stain , pathology , melanoma , medicine , histopathology , atypia , fluorescence in situ hybridization , staining , lesion , acral lentiginous melanoma , dermatology , in situ , biology , chemistry , biochemistry , organic chemistry , cancer research , chromosome , gene
Dermoscopic evaluation of acral volar skin is helpful in differentiating malignant melanomas ( MM ) from benign melanocytic nevi. However, histological diagnosis remains difficult because sufficient evidence of histopathological changes to establish a diagnosis of MM are not easily obtained. The aim of the present study was to evaluate the effective use of fluorescence in situ hybridization ( FISH ) in the diagnosis of acral volar melanocytic lesions, and to determine whether acral volar melanocytic lesions show characteristic molecular biological features of malignant melanoma via FISH . We classified acral volar melanocytic lesions showing junctional findings into three groups: (A) parallel ridge pattern ( PRP ) on dermoscopic examination with melanoma in situ ; (B) PRP with insufficient melanocyte proliferation and atypia to diagnose malignant melanoma using hematoxylin–eosin staining; and (C) junctional nevi. We performed FISH analysis using the same tissue section that was used for hematoxylin–eosin staining. FISH positivity was seen in 80% (4/5) of the group A sections, and in 80% (4/5) of the group B sections. One case in group C was only 0.3% over the established criteria line (63.3% > 63% in RREB 1). Our results suggest that FISH using whole‐slide digital imaging may be useful in the diagnosis of early in situ MM when a typical PRP is observed in an acral volar skin lesion with non‐diagnostic histopathology.