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Role of chemokines and the corresponding receptors in vitiligo: A pilot study
Author(s) -
Yang Lingling,
Yang Sailing,
Lei Jiehao,
Hu Wenting,
Chen Rong,
Lin Fuquan,
Xu Aie
Publication year - 2018
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.14004
Subject(s) - vitiligo , chemokine , hacat , peripheral blood mononuclear cell , immunology , flow cytometry , tumor necrosis factor alpha , epidermis (zoology) , pathogenesis , medicine , biology , inflammation , in vitro , biochemistry , anatomy
To determine the levels and sources of chemokines in the serum and epidermis of vitiligo patients, we examined 80 active patients, 80 stable patients and 40 healthy controls. First, the serum levels of candidate chemokines were measured by Luminex assay, and levels of CCR 5, CXCR 1 and CXCR 3 were measured in peripheral mononuclear cells ( PMBC ) by flow cytometry. Then, the local epidermis levels of elevated chemokines in vitiligo were tested by Luminex. Finally, the mRNA and protein expression levels of elevated chemokines in HaCaT cells stimulated with interferon ( IFN )‐γ or tumor necrosis factor ( TNF )‐α were tested by quantitative real‐time polymerase chain reaction and Luminex. The serum levels of CCL 5, CXCL 8 and CXCL 10 in active vitiligo were significantly elevated compared with those in stable vitiligo patients. Furthermore, the levels of CCL 3 and CCL 4 had weak and positive correlations with the Vitiligo Area Scoring Index. In the peripheral blood of active vitiligo patients, the percentages of CD 3 + CD 8 + CCR 5 + and CD 3 + CD 8 + CXCR 3 + T cells were significantly increased compared with those in stable vitiligo and healthy controls. In the epidermis of lesions, the expression levels of CCL 5 and CXCL 10 in active vitiligo were significantly increased. In addition, the mRNA and protein levels of CCL 5, CXCL 8 and CXCL 10 were significantly elevated in HaCaT cells after stimulation with TNF ‐α or IFN ‐γ. The CCR 5/ CCL 5 and CXCR 3/ CXCL 10 axes may play an important role in the progression and maintenance of vitiligo. Moreover, keratinocytes stimulated with TNF ‐α and IFN ‐γ may be a primary source of CCL 5 and CXCL 10.