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Biochemical effects of the flavanol‐rich lychee fruit extract on the melanin biosynthesis and reactive oxygen species
Author(s) -
Hagiwara Kazuya,
Okura Masae,
Sumikawa Yasuyuki,
Hida Tokimasa,
Kuno Atsushi,
Horio Yoshiyuki,
Yamashita Toshiharu
Publication year - 2016
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.13326
Subject(s) - resveratrol , arbutin , reactive oxygen species , chemistry , tyrosinase , biochemistry , skin whitening , ascorbic acid , polyphenol , cosmeceutical , antioxidant , melanin , hydroquinone , superoxide dismutase , pharmacology , food science , biology , enzyme , active ingredient , microbiology and biotechnology
An ingredient of fruit polyphenol, resveratrol, is known to have an inhibitory effect on melanogenesis. In order to examine the functional differences between resveratrol and other fruit polyphenols, we compared biochemical effects of a resveratrol‐free polyphenol, flavanol‐rich lychee fruit extract ( FRLFE ), with other phenolic compounds including resveratrol. FRLFE as well as hydroquinone and resveratrol suppressed growth of B16F1 melanoma cells more significantly than rhododendrol or arbutin. Resveratrol suppressed mushroom tyrosinase at the lowest concentration (23.0 μmol/L) among the compounds tested. FRLFE and hydroquinone suppressed tyrosinase at almost the same concentration (half maximal inhibitory concentration [ IC 50 ], 83.5 and 94.6 μmol/L, respectively), which was higher than rhododendrol, ascorbic acid and arbutin ( IC 50 , 245, 345 and 421 μmol/L, respectively). Western blot analysis revealed that although resveratrol decreased expressions of tyrosinase and tyrosinase‐related protein 1, FRLFE did not affect their expressions. Both FRLFE and resveratrol suppressed antimycin A‐mediated reactive oxygen species ( ROS ) production in melanocytic cells. Resveratrol‐mediated ROS suppression was inhibited by nicotinamide, a SIRT 1 inhibitor. However, FRLFE ‐mediated suppression was not affected by nicotinamide. Moreover, FRLFE directly decreased superoxide in vitro , as detected by superoxide dismutase‐like scavenging activity assay. These results suggest that FRLFE can protect melanocytes from cytotoxicity caused by an excess amount of melanin and ROS in a different manner from resveratrol.