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Effect of microtubule‐associated protein‐4 on epidermal cell migration under different oxygen concentrations
Author(s) -
Chen Xin,
Zhou Xin,
Mao TongChun,
Shi Xiaohua,
Fan Dongli,
Zhang Yiming
Publication year - 2016
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/1346-8138.13192
Subject(s) - hacat , cell migration , microbiology and biotechnology , cell , wound healing , microtubule , western blot , live cell imaging , chemistry , biology , cell culture , immunology , biochemistry , genetics , gene
After skin trauma, regional epidermal cell migration mediates the re‐epithelialization of the wound surface, which is an important step for wound healing, yet the underlying molecular regulatory mechanism is unclear. In the current study, HaCaT cells were maintained under different oxygen concentrations (1%, 21%, 40% and 65%). Technologies including immunofluorescence staining, wound scratch, transwell invasion, western blot and low‐expression lentiviral vector were utilized to observe the changes in microtubule dynamics and the microtubule‐associated protein ( MAP )4 expression. MAP 4's effect on cell migration under different oxygen concentrations was also studied. The results showed that under hyperoxic (40% and 65%) and hypoxic (1%) conditions, HaCaT cells were able to regulate cell microtubule dynamics by MAP 4, thus promoting cell migration. On the other hand, MAP 4 silencing through targeted sh RNA attenuated HaCaT cell migration under the above oxygen concentrations. These results imply that MAP 4 plays an important role in epidermal cell migration under different oxygen concentrations.